Our new Clear+ tissue clearing method is the sole strategy that delipidates samples without having change in morphology and with minimal impact on structural integrity.
Megatome is actually a vibrating microtome intended to part a wide number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables higher-throughput tissue sectioning with uniform surface area profile, and also small tissue problems and knowledge decline.
Antibodies may well acquire months to diffuse by only some millimeters of tissue, which has a steep labeling gradient from floor to Main.
Megatome is suitable for precision: the blade vibrates at an increased frequency and bigger amplitude array than other microtomes, and contains a unique deflection Handle mechanism.
Clone the project or grab the latest launch. Operating the utility will differ a bit based upon the way you retrieve the undertaking.
Our preformulated EasyIndex Answer raises and homogenize the refractive index of delipidated tissue samples, rendering them fully clear. This permits mild penetration into the sample and makes sure the acquisition of significant-resolution, in-emphasis picture data.
You ought to only need to established the javadocTask house when the undertaking you employ to make Javadoc is non-conventional. By way of example, there may be a endeavor named allJavadoc inside a multi-module
It’s time to up grade your microtome to Megatome. With correct superior-frequency slicing for an unmatched array of sample sizes and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for numerous apps.
Entirely delipidate whole mouse brains or comparably megatomi.com sized samples in only one day with SmartBatch+, or in a single week with our passive clearing package.
Docset creation calls for at minimum two possibilities: the name on the docset and the location in the Javadoc data files to include from the docset.
This will likely create a docset named Sample in The present Listing. Docset creation could be tailored with optional arguments:
Protect avoids the variability of hydrogel embedding and the information reduction from PFA preservation, shielding specimens for numerous rounds of processing.
--displayName: Will established the name as proven in Sprint. This is often handy in case you make a docset with name SampleProject but Show title Sample Job in its place. This environment will default to the value of --title if omitted.
Our novel SHIELD tissue preservation method kinds intramolecular bonds employing polyfunctional, adaptable epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.